Dual chromogenic reporter gene detection in mammalian cells withlacZand arabinofuranosidase

Dual chromogenic reporter gene detection in mammalian cells with lacZ and arabinofuranosidase.

A dual-light reporter system to determine the efficiency of protein–protein interactions in mammalian cells

Methods for determining protein-protein interactions in mammalian cells typically rely on single reporter functions and are susceptible to variations between samples particularly in regard to levels of transcription, processing and translation. A method has been developed for determining protein-protein interactions in mammalian cells, which bypasses these variables confounding single reporter ...

In-depth Characterization of Firefly Luciferase as a Reporter of Circadian Gene Expression in Mammalian Cells

Firefly luciferase (Fluc) is frequently used to report circadian gene expression rhythms in mammalian cells and tissues. During longitudinal assays it is generally assumed that enzymatic substrates are in saturating excess, such that total bioluminescence is directly proportional to Fluc protein level. To test this assumption, we compared the enzyme kinetics of purified luciferase with its acti...

Cover Picture: Detection of mRNA in Mammalian Cells with a Split Ribozyme Reporter (ChemBioChem 6/2006)

The cover picture shows a split RNA reporter strategy for detecting target mRNA in mammalian cells. A tetrahymena group I intron ribozyme reporter is split into two halves, each of which carries a part of the coding sequence of the reporter mRNA, a fragment of the ribozyme, and an antisense sequence complementary to a target mRNA. Both halves bind with the target mRNA and are assembled into an ...

Detection of mRNA in mammalian cells with a split ribozyme reporter.

The detection of mRNA expression in vivo can reveal essential information about basic biology and disease processes. Current methods primarily involve the use of labeled (with fluorophores or radioactive isotopes) antisense oligomers, based on a one-to-one receptor–ligand type of interaction without robust signal amplification, such as molecular beacons. Given that the copy number of a particul...